Figure 4 | Laboratory Investigation

Figure 4

From: Cell-specific effects of TNF-α and IL-1β on alkaline phosphatase: implication for syndesmophyte formation and vascular calcification

Figure 4

TNF-α and IL-1β inhibit TNAP in human MSC-derived chondrocytes. In human chondrocytes cultured in pellets as detailed in ‘Materials and methods’, TNF-α at 1 ng/ml and IL-1β at 0.1 ng/ml decreased the levels of TNAP transcripts after 48 h of treatment, as measured by quantitative PCR (a), and reduce TNAP activity after 7 days of treatment, as revealed by staining of alkaline phosphatase activity of pellet sections (b). *P<0.05 as compared with untreated cells.

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