Figure 1
Disease activity index (DAI) and colon length in mice with dextran sodium sulfate (DSS)-induced colitis. Colitis was induced in wild type (WT) and Mk2−/− mice by oral intake of 4% DSS for 7 days. Bar graphs representing DAI (a) or colon length (b) in control (Con) animals (white bars), DSS-treated animals (gray bars), DSS- and WIN-treated animal (black bars), and single WIN-treated animals (ruled bars). The treatment in every group was: Con: control group in which mice drank water; DSS: the group in which mice drank 4% DSS solution; DSS+WIN: the group in which mice drank 4% DSS solution and received WIN55, 212-2 (WIN55) intraperitoneally (i.p.); WIN: the group in which mice drank water and received WIN55 i.p. DAI was assessed according to the system of Cooper et al28 and Hartmann et al.29 and the total score drifts from 0 (healthy) to 4 (maximal activity of colitis) described in the Materials and Methods section. Colon length (cm) starts from 0.5 cm above the anus to the top of the cecum. Data are shown as means±s.e. (n=6–8 per group, one-way analysis of variance (ANOVA), Tukey’s multiple comparison).*P<0.05 when compared with the Con or WIN group; #P<0.05 when compared with the DSS+WIN group; &P<0.05 when compared with the values of WT mice in the identically treated groups. Mk2, mitogen-activated protein kinase (MAPK)-activated protein kinase-2, also known as MAKAP kinase-2.
