Table 2 Sequence of primers used for real-time PCR in the present study
From: Notch signaling pathway and Cdx2 expression in the development of Barrett's esophagus
Gene name | Amplicon size | Primer sequence | GenBank accession no. | |
|---|---|---|---|---|
Forward | Reverse | |||
GAPDH | 66 bp | 5′-AGCCACATCGCTCAGACAC-3′ | 5′-GCCCAATACGACCAAATCC-3′ | NM_002046 |
Notch1 | 87 bp | 5′-CGCACAAGGTGTCTTCCAG-3′ | 5′-AGGATCAGTGGCGTCGTG-3′ | NM_017617 |
Hes1 | 107 bp | 5′-AGTGAAGCACCTCCGGAAC-3′ | 5′-CGTTCATGCACTCGCTGA-3′ | NM_005524 |
ATOH1 | 79 bp | 5′-GTCCGAGCTGCTACAAACG-3′ | 5′-GTGGTGGTGGTCGCTTTT-3′ | NM_005172 |
Cdx2 | 103 bp | 5′-CAGTCGCTACATCACCATCC-3′ | 5′-CTCCTTTGCTCTGCGGTTCT-3′ | NM_001265 |
MUC2 | 90 bp | 5′-GCTGCTATGTCGAGGACACC-3′ | 5′-GGGAGGAGTTGGTACACACG-3′ | NM_002457 |
