Figure 6 | Laboratory Investigation

Figure 6

From: Estradiol triggers sonic-hedgehog-induced angiogenesis during peripheral nerve regeneration by downregulating hedgehog-interacting protein

Figure 6

E2 enhances Shh signaling in endothelial cells by downregulating HIP expression. (a) Shh and (b) HIP mRNA expression were evaluated in HUVECs, fibroblasts (NIH 3T3), and Schwann cells (SW10) that had been treated with 1 × 10−8 mol/l E2 for 6 h; measurements were performed via qRT-PCR and normalized to endogenous 18S rRNA levels. (c) HUVECs were treated with or without E2 (6 h) and with or without 1 μg/ml Shh (16 h), and then Shh signaling was evaluated via the Gli-luciferase assay. ND indicates not detectable.

Back to article page