Figure 6
Transgelin is expressed in mesangial cells during glomerulogenesis. Kidneys from 6-day-old rats are stained for the mesangial marker OX-7 (a–c) and transgelin (d–f) using immunohistochemistry. During capillary loop stage, OX-7-positive mesangial cells are detectable in the developing glomeruli (a). Transgelin can be detected at a similar localization (d). Later stages of developing glomeruli express OX-7 (b) as well as transgelin (e) in the centre of the glomerulus. In addition, parietal epithelial cells (e, white arrow) and lightly some podocytes also express transgelin. Early mature glomeruli show the typical tree-like OX-7 staining for mesangial cells (c). At this stage, glomerular transgelin staining still decreases (f) whereas transgelin expression of parietal epithelial cells remains prominent (f, white arrow). Developing glomeruli were also analysed by immunofluorescence staining for transgelin (g, green fluorescence staining) together with OX-7 (h, red fluorescence staining). Colocalization of both markers (i) is shown using confocal microscopy.
