Figure 1 | Laboratory Investigation

Figure 1

From: Liver X receptor α-mediated regulation of lipogenesis by core and NS5A proteins contributes to HCV-induced liver steatosis and HCV replication

Figure 1

Effect of HCV proteins and viral replication on intracytoplasmic lipid accumulation. (a) Representative histograms of Nile Red fluorescence obtained by flow cytometry in CHL, CHL–NS5A and CHL–core cells or Huh7 and HCV-G1 cells (see Materials and Methods section for experimental details). Results are expressed as Nile Red relative fluorescence intensity and normalized to control cells. (b) Representative fluorescent images of Nile Red-treated cells. Nuclei were stained with DAPI (blue). Photographs shown are typical results of six independent experiments. (c) Triglyceride assay was performed on cell lysates as indicated in the Materials and Methods. Data are described as the mean values±s.d. of six experiments (*P<0.05 vs CHL or Huh7, #P<0.05 vs CHL–core).

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