Figure 5 | Laboratory Investigation

Figure 5

From: Liver X receptor α-mediated regulation of lipogenesis by core and NS5A proteins contributes to HCV-induced liver steatosis and HCV replication

Figure 5

LXRα does not physically interact with HCV NS5A or core proteins in genomic replicon-containing cells. (a) HCV-G1 cells were processed for double immunostaining using anti-LXRα (green) and anti-core (red) or anti-NS5A protein (red)-specific antibodies. Nuclei were stained with DAPI (blue). The merged image with green and red fluorescence is shown (right). Photographs shown are typical results of six independent experiments. (b) Co-immunoprecipitation of LXRα and HCV proteins. Total lysates from Huh7 and HCV-G1 cells were subjected to immunoprecipitation (IP) using antibodies specific for LXRα, NS5A, or core proteins. Both lysates and precipitates were analyzed via immunoblotting (IB) with the indicated antibodies.

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