Figure 6 | Laboratory Investigation

Figure 6

From: Liver X receptor α-mediated regulation of lipogenesis by core and NS5A proteins contributes to HCV-induced liver steatosis and HCV replication

Figure 6

Effect of PI3K/AKT pathway inhibition on HCV core and NS5A proteins and viral replication-mediated LXRα upregulation. Cultured cells were treated with the PI3K-inhibitor LY294002 (50 μM) for 24 h. (a, b) p-AKT (Ser 473), AKT, and LXRα protein levels in CHL, CHL–NS5A, and CHL–core cells or Huh7 and HCV-G1 cells were analyzed by western blot. Densitometry analysis of specific bands expressed as percentage relative to their controls (100%). β-Actin levels were used as loading control. Molecular weight markers (kDa) are indicated on the left. Photographs are representative of six independent experiments. (c) Bar graphs show LXRα mRNA levels determined by real-time qRT-PCR as indicated. Data are described as the mean values±s.d. of six experiments (*P<0.05 vs CHL or Huh7, #P<0.05 vs nontreated cells).

Back to article page