Table 2 Primer sequences for ChIP assays
Gene | Promoter regions |
|---|---|
SREBP-1c | 5′-GCAGGAAGATGAGACAAATAAAGACA-3′ |
5′-AATGTGGTGAGACCAACAAGCA-3′ | |
FAS | 5′-CTTGCTCCATGGGAATCCA-3′ |
5′-GGGCGACAGAGCAAGACTCT-3′ | |
HPRT | 5′-ATTCGTTCAAATGCATCCTGAAAA-3′ |
5′-GGTCGTAGTCGTAGTGTCAAAAGT-3′ | |
Gene | Coding regions |
SREBP-1c | 5′-CCCATTCCCCAACCTAAAGC-3′ |
5′-TCCTTAAGAGTAAAAAACAGTCATTGCA-3′ | |
FAS | 5′-GAACTCCTTGGCGGAAGAGA-3′ |
5′-CTCGTTGAAGAACGCATCCA-3′ | |
HPRT | 5′-CATTATTTAAACAGGGTAGCAGTAC-3′ |
5′-CAATGTGTGCAGTAGTCAGTAAATC-3′ |
