Figure 7
Nuclear translocation of NOTCH1 is rare, and it occasionally occurs asymmetrically in cultured keratinocytes. (a) Immunofluorostaining of HFS cells using the anti-NOTCH1 antibody. NOTCH1 protein accumulates at the cell–cell interface. Little protein was detected on the free surface or in the nuclei. (b) Immunofluorostaining of HFS cells using the anti-cleaved NOTCH1(Val1744) antibody. This activated form of intracellular domain of NOTCH1 is detected in the nuclei, but only in a very few cells—far <1% of the total population. (c) Immunofluorostaining of Ca9-22 cells transfected with wild-type mouse Notch1 using the anti-NOTCH1 antibody. The antibody crossreacts with mouse Notch1. Endogenous expression of NOTCH1 is observed as fine membranous or cytoplasmic staining. The transfected cells exhibit much stronger expression, facilitating detection. Nuclear localization of the NOTCH1 is observed in one of two neighboring cells, which appear as postmitotic daughter cells. Arrow: postmitotic cell with nuclear NOTCH1; arrowhead: postmitotic cell without nuclear NOTCH1.
