Figure 3

Gefitinib-resistant (GR) cells can survive independently of AXL receptor tyrosine kinase. (a) Western blots of HCC827 or HCC4006 parental and GR cells for the AXL kinase. (b) Western blots examining the effects of AXL depletion, gefitinib treatment, or a combination of AXL depletion and gefitinib or neither in HCC4006 GR3 cells. Cells transfected with negative control (NC) small interfering RNA (siRNA; 10 nM) or AXL-specific siRNA (10 nM) were cultured for 72 h and then untreated or treated with gefitinib (2 μM) for another 48 h.