Figure 1

Immunophenotypic transition during the culture of sorted subpopulations. (a) MWCL-1 cells were stained with CD20. The sorted CD20+ and CD20− cells were independently cultured, and the change of CD20 expression was examined for 132 h. (b) MWCL-1 cells were stained both CD20 and CD138. Most MWCL-1 cells were classified into three subpopulations: CD20− CD138−, CD20+ CD138−, and CD20+ CD138+. The sorted CD20− CD138− cells were cultured, and the change of CD20 and CD138 expression was examined. (c) The sorted CD20+ CD138− and CD20+ CD138+ cells were cultured, and CD20 and CD138 expression levels were examined.