Figure 3 | Laboratory Investigation

Figure 3

From: Characterization of subpopulation lacking both B-cell and plasma cell markers in Waldenstrom macroglobulinemia cell line

Figure 3

In vitro colony formation. When a total of 5 × 104 cells were plated in methylcellulose-containing medium, colonies were obtained from CD20 CD138 subpopulation but not from CD20+ CD138 and CD20+ CD138+ subpopulations. When a total of 1 × 105 cells were plated, the number of colonies obtained from CD20 CD138 subpopulation was significantly higher than that of CD20+ CD138+ and CD20+ CD138 subpopulations. The number of colonies was counted on day 23 when a total of 1 × 105 cells were plated, and on day 35 when a total of 5 × 104 cells were plated. *P<0.05 when compared with CD20 CD138 cells.

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