Figure 2 | Laboratory Investigation

Figure 2

From: Evaluating the repair of DNA derived from formalin-fixed paraffin-embedded tissues prior to genomic profiling by SNP–CGH analysis

Figure 2

Validation of DNA copy number amplifications detected in restored FFPE samples. DNA aberrations were validated by immunohistochemistry (IHC) and in situ hybridization. Tumor samples Q590 (a) and 540 (C) both exhibited amplification of 17q12-q21, encompassing the ERRB2 gene (arrow). These were validated by chromogenic in situ hybridization (b) or IHC (d). Tumor 007 (e) harbored an amplification at 7p11.2 encompassing the EGFR gene; overexpression of EGFR protein was validated by IHC (f; scored as 3+ in 100% of cells).

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