Table 1 Specific mouse primers for real-time RT-PCR amplifications

From: A small interfering RNA targeting Lnk accelerates bone fracture healing with early neovascularization

 

Primer sequences (5′-3′)

 

Gene name

Forward

Reverse

PCR product size (bp)

mLnk

ATTTTCCTCCCCTCCAGAGA

GACAGCCTTGTGTGTGTTGG

86

mCD31

TCCCCACCGAAAGCAGTAAT

CCCACGGAGAAGTACTCTGTCTATC

224

mVE-Cad

GAGCTAAGAGGACCCTCTGCTACTC

TGGGCCTCTTTGTGTCTGTATG

369

mAng-1

ATCTTGATAACCGCAGCCAC

TGTCGGCACATACCTCTTGT

166

mVEGF

TTACTGCTGTACCTCCACC

ACAGGACGGCTTGAAGATG

189

mOC

CTGACCTCACAGATCCCAAGC

TGGTCTGATAGCTCGTCACAA

187

mCol1A1

CAATGGTGAGACGTGGAAAC

GGTTGGGACAGTCCAGTTCT

107

mBMP-2

TCACTTATAGCCGCATTATCTTCTTC

TTGGTTTATCCATGAGGCTAACTG

73

mBMP-4

GCCATTGTGCAGACCCTAGT

TCAGTTCAGTGGGGACACAA

73

mRunx2

CGGCCCTCCCTGAACTCT

TGCCTGCCTGGGATCTGT

75

mOsx

ACTGGCTAGGTGGTGGTCAG

GGTAGGGAGCTGGGTTAAGG

135

mALP

GACTGACCCTTCGCTCTCCG

TCCATCTCCACTGCTTCATG

156

mGAPDH

GTGAGGCCGGTGCTGAGTATG

AGGCGGCACGTCAGATCC

484

  1. Abbreviations: mLnk: mouse Lnk; mCD31: mouce CD31; mVE-Cad: mouse vascular endothelial cadherin; mAng-1: mouse 1 angiopoietin-1; mVEGF: mouse vascular endothelial growth factor; mOC: mouse osteocalcin; mCol1A1: mouse collagen 1 alpha 1; mBMP-2: mouse bone morphogenetic protein-2; mBMP-4: mouse bone morphogenetic protein-4; mRunx2: mouse runt-related transcription factor 2; mOsx: mouse osterix; mALP: mouse alkaline phosphatas; mGAPDH: mouse glyceraldehydes-3-phosphate dehydrogenase; bp: base pair.
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