Figure 5

RT-PCR for FGF4 and its related genes. (a) Gene expression analysis of FGF family. Gene expression in tumor samples and normal skin fibroblasts was analyzed by RT-PCR. GAPDH was used as an internal control. (b) Gene expression analysis with tumor samples and fibroblasts. Gene expression in tumor samples and normal skin fibroblasts was analyzed by RT-PCR. GAPDH was used as an internal control. (c) Gene expression analysis with in vitro cultured cells. Gene expression in four groups of in vitro cultured cells (fibroblast-mixed ALDH1^high cells, ALDH1^high only cells, fibroblast-mixed ALDH1^low cells, ALDH1^low only cells) was analyzed by RT-PCR. GAPDH was used as an internal control. (d) Immunostaining for FGF4 and FGFR2. FGF4 (upper panels) and FGFR2 (lower panels) were detected by immunostaining with specific antibodies. × 200 magnification.