Figure 2 | Laboratory Investigation

Figure 2

From: Hemoglobin-associated oxidative stress in the pericardial compartment of postoperative cardiac surgery patients

Figure 2

Hemoglobin and methemoglobin in postoperative PCF. Samples collected from the same patient during surgery (0 h) and post surgery (4, 12, 24, and 48 h) were immediately centrifuged at 500 g for 15 min, supernatants collected, and centrifuged again to remove cells and large debris at 1500 g for 10 min. (a) The oxyhemoglobin and (b) methemoglobin levels are visualized for each patient at the corresponding time after surgery as measured by spectrophotometry. (c) The methemoglobin/oxyhemoglobin ratio (MetHb/OxyHb) is shown to determine time points of relatively greater oxidative stress compared with the 0 h control. The mean values are represented by a horizontal bar. Data from 31 patients. *P<0.05, **P<0.005.

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