Figure 5 | Laboratory Investigation

Figure 5

From: Hemoglobin-associated oxidative stress in the pericardial compartment of postoperative cardiac surgery patients

Figure 5

Postoperative pericardial fluid (4 h) primes neutrophils for increased oxidative burst. Pericardial fluid was centrifuged at 1500 g and then diluted to 2.5, 5, and 10% in XF DMEM. It was then added to freshly isolated and plated healthy donor neutrophils 1.5 h before the assay. (a) Baseline OCR was measured in neutrophils before inhibition of mitochondrial respiration with antimycin A (10 μ M) and induction of the oxidative burst with phorbol 12-myristate 13-acetate (PMA, 100 ng/ml). (b) The area under the curve (AUC) during the 48-min oxidative burst response was calculated and plotted as a function of PCF dose. *P<0.05, **P<0.005. Three to five replicated wells per group.

Back to article page