Figure 6
Quantitation of F2α Isoprostanes and prostaglandins in postoperative PCF by LCMS/MS. Lipids were extracted from postoperative pericardial fluid using solid-phase extraction columns as described in the Materials and Methods and analyzed with mass spectrometry. (a) 8-iso-prostaglandin F2α concentrations and (b) prostaglandin F2α concentrations were assessed in 0, 4, 12, 24, and 48 h postoperative PCF patient samples. (c) Regression analysis of F2α isoprostanes plotted against prostaglandins over all time points. Data represented as individual patient values plus with the mean represented by a horizontal bar at 0 h (n=13), 4 h (n=22), 12 h (n=23), 24 h (n=12), and 48 h (n=8). Statistical significance was determined using one-way ANOVA followed by Tukey’s post hoc analysis, with P<0.05 indicating statistical significance. *P<0.05, **P<0.005.
