Figure 2
PBCre4 efficiently targets Foxa1-positive prostate epithelium. Immunofluorescence on anterior prostate tissue dissected from PBCre4/Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J mice (PBCre4/Tomato mice) was performed to determine the extent to which PBCre4 targets Foxa1-positive prostate epithelium. The presence of membrane bound GFP in PBCre4/Tomato mice is indicative of a cre-mediated recombination event. Panels a (DAPI), b (Foxa1), c (GFP), and d (merge) show that Foxa1-positive prostate epithelium in the anterior prostate are efficiently targeted by PBCre4 in the AP. In terms of percentage, a range of GFP-positive cells where Cre was activated was detected in the anterior prostate (AP), with the distal tips and base of the AP being the most affected (see Supplementary Data for analysis of other prostate lobes).
