Figure 4
PBCre4-mediated Foxa1 knockout is mosaic in nature and results in increased epithelial proliferation. Panel a: Foxa1 immunostaining of anterior, dorsal, lateral, and ventral prostate tissue dissected from 24-week-old control Foxa1loxp/loxp mice and age-matched PBCre4/Foxa1loxp/loxp mice reveal Foxa1 knockout is mosaic in nature, in keeping with PBCre4/Tomato mice cre mapping studies. Panel b: Ki67 staining reveals an increase in the number of proliferating cells following Foxa1 knockout in 24-week-old PBCre4/Foxa1loxp/loxp mice (see Supplementary Data for graphical representation of Ki67 index). Panel c: dual immunofluorescence for Foxa1 (red) and Ki67 (green) shows that detection of Ki67 staining is not limited to Foxa1-negative cells in the anterior prostate (see merged panel in c). White arrows point to Foxa1-negative, Ki67-positive epithelium, while yellow arrows point to Foxa1-negative epithelium that is Ki67 positive.
