Figure 2
Validation and performance of the tissue quality index (TQI) on the time to fixation breast cancer (TFBC), the normal breast tissue (NBT), and Italian breast cancer (IBC) series. (a) Linear regressions between increasing time to fixation (log2 transformed) and the differences of AQUA scores of the TQI markers as performance measurement on an independent built of the TFBC. Higher values correspond with shorter time to fixation. The dotted line shows the 95% confidence interval (CI) of the regression line. (b) TQI pairs on each time-point of the NBT series. (c) Chi-squared analysis of the different TQI components. Although each marker combination by itself shows a significant correlation to increasing cold ischemic time, the combination of the separate TQI components facilitates the identification of a larger number of samples, which may have compromised tissue quality. (d) The TQI performance on the IBC series where special fixation conditions appears to result in significantly less epitope degradation.
