Table 1 Antibodies tested for the TQI
| Â | Â | Antibody | Â |
|---|---|---|---|
Symbol | Description | Clone/Isotype | Supplier |
Markers of Cold Ischaemia | |||
 ACTB | Beta-Actin | 13E5/IgG | Cell Signaling Technology |
 TUBB | Beta-Tubulin | pF3/IgG | Cell Signaling Technology |
 GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | 14C10/IgG | Cell Signaling Technology |
 HIST4 | Histone 4 | L64C1 | Cell Signaling Technology |
 HIST3 | Histone 3 | 96C10/IgG1, kappa | Cell Signaling Technology |
 LMNA/C | Lamin A/C | Polyclonal | Cell Signaling Technology |
 LDHA | Lactat Dehydrogenase | IgG, C4B5 | Cell Signaling Technology |
 ERalpha | Estrogen Receptor alpha | SP1/IgG | Thermo Scientific |
 CK | Cytokeratin | AE1/AE3/IgG1 | DAKO |
 CK | Cytokeratin | Polyclonal | DAKO |
 ERK1/2 | P44/42MAPK (Erk1/2) | 137F5, IgG | Cell Signaling Technology |
 p53 | Anti-Human p53 protein | IgG2b. DO-7 | DAKO |
Markers of Hypoxia | |||
 CCND1 | Cyclin D1 | IgG/SP4 | Thermo Fisher Fremont |
 Caspase | Cleaved Caspase 3 (Asp175) | Polyclonal | Cell Signaling Technology |
 HIF1 | Hypoxia Inducible Factor 1 | Polyclonal | Novus Biological |
 AKAP13 | A-kinase anchoring protein 13 | IgG2a/ZX-18 | Santa Cruz Biotechnology |
 CDC42 |  | IgG3/B-8 | Santa Cruz Biotechnology |
 CCNB1 | Cyclin B1 | GNS-11/IgG2 | BD Biosciences |
 HIF-2alpha | Hypoxia inducible factor-2α | ep190b/IgG1 | Abcam |
 CA9 | Carbonic Anhydrase IX | Polyclonal(aa581-592) | Lifespan Biosciences |
Markers of phosphorylated proteins | |||
 pAKT 473 | phospho-Akt (ser473) | D9E/IgG | Cell Signaling Technology |
 ERK1/2 | Phospho-p44/43MAPK (Erk1/2) (Thr292/Tyr204) | IgG | Cell Signaling Technology |
 pER | Phospho-Estrogen Receptor alpha (Ser118) | 16J4/IgG2b | Cell Signaling Technology |
 Anti-Phosphotyrosine | 4G10 Anti-Phosphotyrosine | IgG2b | Millipore |
 Anti-Phosphotyrosine |  | p-Tyr-100 | Cell Signaling Technology |
 pHSP27 (pS78) | Phosphorylated Heat Shock Protein 27 | Y175 | Epitomics |
 pHer2 (Tyr1248) | Phospho-Her2/ErbB2 (Tyr1248) | PN2A | Thermo Scientific |
 Phospho-Stat3 (Tyr705) | Phospho-Stat3 (Tyr705) | D3A7/IgG | Cell Signaling Technology |
 p-S6 Ribosomal Protein (Ser235/236) | Phospho-S6 Ribosomal Protein (Ser235/236) | D52.2.2E/IgG | Cell Signaling Technology |
 Phospho-Jak2 (Tyr1007/1008) | Phospho-Jak2 (Tyr1007/1008) | Polyclonal | Cell Signaling Technology |
 Phospho-Met (Tyr1234/1235) | Phospho-Met (Tyr1234/1235) | IgG | Cell Signaling Technology |
 Phospho-Sapk/Jnk | Phospho-Sapk/Jnk | IgG | Cell Signaling Technology |
 Phospho mTor (Ser2448) | Phospho mTor (Ser2448) | 49F9/IgG | Cell Signaling Technology |
Markers of posttranslational modification | |||
 Sumo1 | small ubiquitin related modifier 1 | Y299/IgG | Abcam |
 Acetylated-Lysine | Proteins posttranslat. Modified by acetylation | Polyclonal, purified | Cell Signaling Technology |
 NEDD8 | Neural precursor cell-expr. devel. Downreg. protein9 | IgG, 19E3 | Cell Signaling Technology |
