Figure 2 | Laboratory Investigation

Figure 2

From: Nuclear hormone receptor LXRα inhibits adipocyte differentiation of mesenchymal stem cells with Wnt/beta-catenin signaling

Figure 2

Expression of adipocyte-related genes. (a and b) Murine MSCs from wild-type (WT) and LXR-null (LXRKO) mice were incubated in adipogenic medium for 9 days. Total RNA was analyzed by quantitative RT-PCR for mRNA expression of PPAR-gamma (a) and fatty acid synthase (b) on day 9. Fold changes vs WT group. Data are mean±s.e.m. (n=4). *P<0.05 vs WT group. (c and d) Murine MSCs overexpressing GFP alone (mMSC/GFP) and mMSCs overexpressing GFP and LXRα (mMSC/LXRα/GFP) were incubated in adipogenic medium for 9 days. Total RNA was analyzed by quantitative RT-PCR for mRNA expression of PPAR-gamma (c) and fatty acid synthase (d) on day 9. Fold changes vs mMSC/GFP group. Data are mean±s.e.m. (n=4). *P<0.05 vs mMSC/GFP group.

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