Figure 5
From: Recombinant human MFG-E8 ameliorates colon damage in DSS- and TNBS-induced colitis in mice
rhMFG-E8 decreases the expression of pro-inflammatory cytokines in mice with DSS- and TNBS-induced colitis. The descending colons of mice exposed to DSS (n=5/group) or transverse colons of mice exposed to TBNS (n=10/group) were collected at the end of the observation period, submitted to RNA extraction, cDNA synthesis, and qPCR amplification. (a) The expression of TNF-α, IL-1β, and IL-6 was significantly elevated in mice exposed to DSS and treated with vehicle, compared with control mice, and treatment with rhMFG-E8 was associated with a dose-dependent reduction in the expression level of these pro-inflammatory cytokines. (b) The expression of TNF-α, IL-1β, and IL-6 was also elevated in mice exposed to TNBS and treated with vehicle, compared with control mice, and treatment with rhMFG-E8 was also associated with a dose-dependent decrease in the expression level of these pro-inflammatory cytokines. Ctrl=control; Veh=vehicle; 60=rhMFG-E8 60 μg/kg/day; 120=rhMFG-E8 120 μg/kg/day; *P<0.05 vs control; #P<0.05 vs vehicle.
