Figure 3 | Laboratory Investigation

Figure 3

From: Dipeptidyl peptidase-4 deficiency protects against experimental diabetic nephropathy partly by blocking the advanced glycation end products-receptor axis

Figure 3

ICAM-1 gene expression in the kidneys (a) and glomerular area (b) of Control, DPP-4-deficient, STZ or DPP-4-deficient STZ rats at 9 weeks old. (a) Total RNAs were extracted from the kidneys of Control, DPP-4-deficient, STZ or DPP-4-deficient STZ rats with RNAqueous-4PCR kit. Quantitative real-time RT-PCR was performed. Data were normalized by the intensity of β-actin mRNA-derived signals. *P<0.05 compared to the value with STZ rats. N=3 for Control rats. N=4 for DPP-4-deficient rats. N=4 for STZ rats. N=3 for DPP-4-deficient STZ rats. (b) The kidney sections were stained with hematoxylin and eosin for light microscopic analysis. Glomerular area was measured using Image J software. Fifteen glomeruli in each sample were evaluated. Upper panel shows the representative microphotographs. Lower panel shows the quantitative data. **P<0.01 compared to the value with STZ rats. N=4 for Control rats. N=4 for DPP-4-deficient rats. N=4 for STZ rats. N=3 for DPP-4-deficient STZ rats.

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