Figure 4
From: Development and characterization of human-induced pluripotent stem cell-derived cholangiocytes
Expression of cholangiocyte proteins. (a) Western blotting shows enrichment of multiple, functionally relevant biliary proteins in induced pluripotent stem cell (iPSC)-derived cholangiocytes (iDCs) including acetylated α-tubulin, AQP-1, ASBT, SSTR-2, cytokeratin-7 (CK-7), and CK-19, while c-Met was not significantly detected. (b) iDCs were stained for CK7 and CK19 by immunofluorescence (left panels). HepG2 hepatocytes showed no staining for either marker (right panels). (c) Flow cytometry analyses of choalngiocyte markers, including CK-7, CK-19 and CFTR (panels I, II and III, respectively) and hepatic endoderm marker EpCAM (panel IV). Scale bars, 50 μm.
