Figure 1 | Laboratory Investigation

Figure 1

From: TGFβ1 exacerbates blood–brain barrier permeability in a mouse model of hepatic encephalopathy via upregulation of MMP9 and downregulation of claudin-5

Figure 1

The BBB is disrupted in the later stages of HE. (a) Evan’s blue dye permeability assay of AOM mice at indicated time points following AOM injection (n=4). Permeability was measured by measuring absorbance of Evan’s blue dye (620 nM). (b) Representative pictures of brains from mice that had no Evan’s blue dye extravasation (untreated), mice infused with Evan’s blue dye after AOM treatment from 0–12 h, and mice infused with Evan’s blue dye 18 h after AOM injection. (c) Immunofluorescence in vehicle and AOM cortex for albumin (red) and DAPI (blue). Data in the permeability assay are reported as mean±s.e.m. *=P<0.05 compared with 0 h mice.

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