Figure 9
The expression of exogenous Plin5 induces gene expression of GCL subunits in HSC, likely by stimulating the transcriptional activity of Nrf2. Passaged HSC were transducted with LV-Plin5-YFP (LV-Plin5), or LV-YFP, or no transduction (Mock). After selection with puromycin, some of the cells were transfected with the plasmid p8xARE-Luc for luciferase assays. Cells were cultured for additional 24 h. (a). Real-time PCR assays. *P<0.05 vs mock cells (n=3); (b) western blotting analyses. Representatives were presented from three independent experiments. β-tubulin was used as an invariant control for equal loading; (c) luciferase activity assays. *P<0.05 vs mock cells (n=6). The floating schema denoted the plasmid p8x-ARE-Luc in use for transfection to the system.
