Figure 7
The effects of LDE225 and itraconazole on expression of Gli-1, Gli-2, and ABCC1 in Huh-7-DN subpopulation. Huh-7-DN cells were exposed to LDE225 or itraconazole at various concentrations as indicated for 24 h. mRNA levels of Gli-1, Gli-2, and ABCC1 in Huh-7-DN subpopulation were determined by quantitative RT-PCR, using untreated Huh-7 cells as a control. Protein levels of Gli-2 and ABCC1 were determined by western blot analysis, using β-actin as a loading control. Neither LDE225 nor itraconazole effectively suppressed Gli-1 mRNA expression (a and d), whereas LDE225 (10 μM) significantly reduced Gli-2 mRNA expression (b and e). Both LDE225 and itraconazole treatment inhibited ABCC1 mRNA expression in a dose-dependent manner, and a maximum inhibitory effect was observed at 10 μM (c and f). Gli-2 protein levels started to decrease from 5 to 10 μM and ABCC1 protein levels were significantly reduced at 10 μM when exposure to LDE225 or itraconazole in Huh-7-DN subpopulation (g and h). *P<0.05, **P<0.01 compared with untreated Huh-7-DN subpopulation.
