Figure 8
The effects of LDE225 and itraconazole on expression of Gli-1, Gli-2, and ABCC1 in Huh-7-trans subpopulation. Huh-7-trans cells were exposed to LDE225 or itraconazole at various concentrations as indicated for 24 h. mRNA levels of Gli-1, Gli-2, and ABCC1 in Huh-7-trans subpopulation were determined by quantitative RT-PCR, using untreated Huh-7 as a control. Protein levels of Gli-2 and ABCC1 were determined by western blot analysis, using β-actin as a loading control. Similar to Huh-7-DN subpopulation, LDE225 or itraconazole did not reduce Gli-1 mRNA expression (a and d). LDE225 or itraconazole could not only significantly inhibit Gli-2 mRNA, but also ABCC1 mRNA expression (b, c, e and f). ABCC1 protein levels started to decrease when LDE225 or itraconazole concentration reached to 1.0 μM (g and h). *P<0.05, **P<0.01 compared to untreated Huh-7-trans subpopulation.
