Figure 4
Lm-332 attenuates RANK expression after RANKL stimulation. BMMs and RAW cells were cultured in plates coated with the indicated concentrations of Lm-332 in the presence of M-CSF (20 ng/ml) and RANKL (50 ng/ml) for 4 days. (a) mRNA expression of Rank was analyzed by quantitative real-time PCR. The expression was normalized to B2m, and the expression level is shown relative to the control (designated as 1) for comparison; bars, ±s.d. *P<0.05. **P<0.01 by ANOVA with Tukey's test. (b) At each time point, mRNA expression of Rank was analyzed. Expression was normalized to B2m, and the expression level is shown relative to the day 0 level (designated as 1) for comparison. **P<0.01 compared to BMMs cultured in Lm-332-coated plates in the presence of RANKL by ANOVA with Tukey's test.
