Figure 1

mDia1/miR-146a double knockout mice develop age-related anemia and ineffective erythropoiesis. (a) Complete blood count of different time points of mDia1/miR-146a double wild type (DWT), mDia1 or miR-146a single knockout (KO), and double knockout (DKO) mice. DWT, n=6; miR-146a KO, n=6; mDia1 KO, n=5; DKO, n=7. (b, c) Flow cytometric analysis of bone marrow cells from indicated mice at 1 year old. Total bone marrow cells (b) and absolute Ter119⁺ erythroid cells (c) from femur and tibia were presented. DWT, n=6; miR-146a KO, n=4; mDia1 KO, n=5; DKO, n=5. (d) Kaplan–Meier survival analysis of the indicated mice. DWT, n=14; miR-146a KO, n=8; mDia1 KO, n=6; DKO, n=11. Both males and females were included. (e, f) The Ter119⁺ erythroid cells in c were analyzed by CD44 levels and forward scatter to define various developmental stages of erythroblasts (Pro-: proerythroblasts, Baso-: basophilic erythroblasts, Poly-: polychromatic erythroblasts, Ortho-: orthochromatic erythroblasts, reticulocytes (Retic-) and mature red blood cells (RBC)). Quantification of the percentages and absolute cell numbers of erythroblasts at each stage of differentiation were presented in e and f, respectively. DWT, n=6; miR-146a KO, n=4; mDia1 KO, n=5; DKO, n=5.