Figure 6
In vitro IL-6-treated bone marrow erythroblasts share the same gene expression profiles as the erythroblasts from DKO mice. (a, b) Volcano plot of microarray data, as generated by clustering based on the genes that were significantly enriched (red dots) or depleted (green dots) (fold change >2; ANOVA P<0.05) in IL-6-treated (a) or DKO erythroblasts (b) over their controls. Representative up or downregulated genes are pointed out. I represents proerythroblasts, II+III represent basophilic and polychromatic erythroblasts, IV represents orthochromatic erythroblasts. (c) Venn diagram defining significantly upregulated genes in in vitro IL-6-treated erythroblasts and erythroblasts from different developmental stages in DKO mice and compared with their corresponding controls. The numbers of overlapped genes are also shown. (d) Venn diagram showing the overlapping signaling pathways generated by PANTHER pathway analysis among the indicated groups (d). 30 signaling pathway were enriched both in Day 2 IL-6-treated erythroblasts and DKO erythroblasts. (e) The percentage of gene hits against total numbers of Pathway hits and gene numbers in specific pathway are illustrated by color scales. (f) Model of the age-related bone marrow microenvironment in the pathogenesis of ineffective erythropoiesis in del(5q) MDS.
