Figure 1
From: Cholera toxin B suppresses allergic inflammation through induction of secretory IgA
Cholera toxin B (CTB) inhibits the potential of dendritic cells (DCs) to induce allergic sensitization. DCs were pulsed overnight by phosphate-buffered saline (PBS), ovalbumin (OVA) (100 μg/ml), OVA+CTB (10 μg/ml), or CTB (10 μg/ml). One million DCs were instilled in the airways of naïve mice. After 10 days, the mice were exposed to three OVA aerosols. At 1 day after the last aerosol, the mice were killed and lung lavages were taken. (a) The cellular composition of the BAL was determined by flowcytometry. (b) Mediastinal lymph node (MLN) cells were cultured with OVA (10 μg/ml) for 4 days. Cytokine production was determined by ELISA (enzyme-linked immunosorbent assay). (c) Lungs were fixed with OCT and snap-frozen. Sections of 3-μm thickness were cut and stained with hematoxylin and eosin. The number of CD4+CD25+FoxP3+, pDC, and B cells in BAL (stained for CD4, CD25, FoxP3, CD19, B220, and 120G8) (d) and the number of CD4+CD25+FoxP3+ T cells in MLN (e) were determined by flowcytometry. Data are mean±s.e.m, n=4 to 6 mice in each group. *P<0.05, **P<0.01, ***P<0.001. Data from one representative experiment out of three are shown.
