Figure 4
From: Cholera toxin B suppresses allergic inflammation through induction of secretory IgA
Cholera toxin B (CTB)-induced B cells transfer suppression of allergy by secretory IgA. Mice were injected with pulsed bone marrow-derived dendritic cells and challenged as described in Figure 1 legend. Mediastinal lymph node (MLN) B cells were isolated by CD19-microbeads and intravenously injected (5 × 106) in previously ovalbumin (OVA)-sensitized wild-type (WT) or pIgR−/− mice at day 10. After 2 days, the mice were challenged and BAL cell differential counts were determined by flowcytometry (a). MLN cells were cultured with OVA (10 mg/ml) for 4 days. Cytokine production was determined by ELISA (enzyme-linked immunosorbent assay) (b: WT; c: pIgR−/−). Data are mean±s.e.m, n=4 to 6 mice in each group. *P<0.05, **P<0.01. Data from one representative experiment out of two or three are shown.
