Figure 2
Antibody and T-cell responses in Broncho-Vaxom (BV)-treated mice. Mice were treated daily with BV or vehicle (vcl) from day –1 to day 13, sensitized with LACK/aluminum hydroxide (alum) on days 0 and 7, and challenged with daily aerosols of LACK or phosphate-buffered saline (PBS) from days 17 to 21. Mice were analyzed on day 23. (a) Bronchoalveolar lavage fluid (BALF) and lung cells were prepared from individual mice and analyzed by fluorescence-activated cell sorting (FACS) upon staining with anti-CD4 and anti-T1/ST2 monoclonal antibodies (mAbs). Data show the number of T1/ST2+ CD4+ cells in individual mice and are expressed as mean±s.e.m., n=8–12 mice per group pooled from two experiments. (b) Protein extracts were prepared from the lungs of individual mice and analyzed for interleukin (IL)-4, IL-5, IL-6, IL-10, and IL-13 content by cytometric bead array (CBA), and for eotaxin content by enzyme-linked immunosorbent assay (ELISA). Data are shown for individual mice with bars indicating the mean value, n=8–12 per group pooled from two experiments. (c) Levels of LACK-specific IgE, IgG1, and IgG2a in the serum of the indicated mice were measured by ELISA. Data are expressed as mean±s.e.m., n=30 mice per group pooled from five experiments. ***P<0.001; **P<0.01; *P<0.05; NS, nonsignificant.
