Figure 3
CXCR3 (chemokine (C-X-C motif) receptor 3) and CCR5 (chemokine (C-C motif) receptor 5) are upregulated on the surface of antigen-specific CD4+ T cells in a stepwise fashion following Chlamydia trachomatis infection. Wild-type CD90.1 Chlamydia-specific CD4+ T cells were transferred intravenously into CD90.2 hosts. Mice were challenged in the genital tract with C. trachomatis the following day. At 7 days after infection, the tissues were isolated and analyzed by flow cytometry. Chlamydia antigen-specific cells were identified by CD4+CD90.1+ gating and assessed for surface expression of CXCR3 and CCR5. Shown is a representative plot from three independent experiments.
