Figure 4
Chlamydia-specific CD4+ cells lacking CXCR3 (chemokine (C-X-C motif) receptor 3) and/or CCR5 (chemokine (C-C motif) receptor 5) are unable to traffic to the genital mucosa. (a) Wild-type, CXCR3−/−, or CCR5−/− CD90.2+ Chlamydia-specific T cells were transferred into CD90.1+ host mice. Mice were challenged the following day with Chlamydia trachomatis in the genital tract. At the time points indicated, the genital tract was harvested and cells prepared for flow cytometry. The total number of antigen-specific T cells was also quantified. Each data point represents the total number of cells from three mice for each genotype. The figure represents one experiment of two conducted. (b) CD90.2+ wild-type, CXCR3−/−, CCR5−/−, or CXCR3−/− CCR5−/− transgenic CD4+ T cells were transferred into CD90.1+ host mice and infected the following day with C. trachomatis. At 8 days following infection, the tissues were harvested and prepared for flow cytometry. Shown is a representative cytometry plot from one experiment of three conducted of the draining lymph node (top panel) and genital tract (bottom panel). The host CD4+ T cell population is shown in gray and the antigen-specific CD4+ T cells are shown in black. The values presented are a percentage of total live cells. (c) Absolute total numbers of Chlamydia-specific CD4+ T cells in the genital tract were quantified from the experiment in b. (d) Cells isolated from the draining lymph node were pre-gated for CD90.2+ and CD4+ populations and examined for surface staining of the activation markers CD45Rb and CD62L. Shown is a representative plot from one of two independent experiments. *P<0.05.
