Figure 5
Chemokine receptor-deficient Chlamydia-specific CD4+ T cells are unable to compete with wild-type cells in homing to the genital tract. (a) A schematic of the competitive homing assay is shown. Equal numbers of antigen-specific CD4+ T cells from CD45.2/CD90.1 wild-type mice and CD45.2/CD90.2 wild-type, CXCR3−/−, CCR5−/−, or CXCR3−/− CCR5−/− mice were transferred into CD45.1/CD90.2 host mice. The following day, mice were infected in the genital tract with Chlamydia trachomatis. At 7 days after infection, tissues were harvested and prepared for flow cytometry. The strategy for gating is illustrated. We first gated CD4- and CD45.2-positive cells. We then compared the percentage of the gated cells that were CD90.1 or CD90.2 positive. Shown is a representative plot comparing the homing to the genital tract between the wild-type and wild-type cell mix and between CXCR3−/−CCR5−/− and wild-type mix. Shown is one representative of four independent experiments. (b) We calculated a migration index for each cell mix using the percentage of CD90.2/CD90.1-positive cells present in the draining lymph node (top) and the percentage of the CD90.2/CD90.1-positive cells in the genital tract (bottom). Error bars represent s.d. and *P<0.05.
