Figure 2
Mast cells inhibit F. tularensis LVS and SCHU S4 induction of active caspase-3. Macrophages, mast cells, co-cultures, or macrophages and 25 ng ml−1 of rIL-4 were infected with LVS (MOI=100) or SCHU S4 (MOI=100). Active caspase-3 was evaluated by flow cytometry (mast cells, macrophages, or co-cultures) at 3 and 24 h. Expression of active caspase-3 in LVS or SCHU S4-infected macrophages was compared with infected macrophages in co-culture with mast cells or with addition of rIL-4 (25 ng ml−1). Unpaired t-test, *P<0.05. Within co-cultures, cells were gated on CD11b+ for macrophage analysis; 10,000 events were acquired. Results are representative of three independent experiments. LVS, Live Vaccine Strain; rIL-4, recombinant interleukin-4; MOI, multiplicity of infection.
