Figure 2
Phenotype of Escherichia coli heat-labile enterotoxin B subunit (EtxB)-positive cells in lymphoid tissues and peripheral blood. Mice were treated with Alexa 647-labeled EtxB on four consecutive days. On day 1 post-treatment, cervical lymph nodes (CLNs) and spleen were removed and enzymatically digested. The resultant cell suspensions were stained with a panel of antibodies specific for lineage markers and analyzed by flow cytometry. (a) Representative plots of Alexa 647-labeled EtxB+ and lineage marker+ cells from the CLNs and spleen of mice treated with labeled or unlabeled EtxB. (b) The frequency of Alexa 647-labeled EtxB+ cells within each cell subset was then determined (n=3). Peripheral blood was also removed at various time points following Alexa 647-labeled EtxB treatment and the peripheral blood mononuclear cells (PBMCs) isolated, and analyzed by flow cytometry for the (c) presence of Alexa 647-labeled EtxB+ cells (n=6) and (d) stained with a panel of antibodies specific for lineage markers (n=6). Error bars represent s.e.m.
