Figure 5
From: Resistance to HSV-1 infection in the epithelium resides with the novel innate sensor, IFI-16
p204 drives innate resistance to HSV but not VSV in the cornea. (a) WT and CD118−/− corneal sensitivity to VSV was compared 48 h pi following infection with 1,000 p.f.u. per eye VSV by RT-PCR. *P<0.05 comparing WT with CD118−/−. (b) WT mice were transfected with either nonspecific siRNA or targeted to p204. Twenty-four hours after transfection, corneas were scarified and infected with VSV. Corneas were isolated 48 h pi and VSV levels were then evaluated by RT-PCR. Expression was normalized to β-actin levels. Bars represent 2 independent experiments of 2–4 corneas per group. HSV, herpes simplex virus; pi, post infection; RT-PCR, reverse transcriptase-PCR; siRNA, small-interfering RNA; WT, wild type; VSV, vesicular stomatitis virus.
