Figure 10
Regulatory T cells in accepted lung grafts. (a) Flow cytometric analysis of Foxp3-expressing CD4+ T cells in (left) lungs derived from naive B6 mice (3.01±0.46% (s.e.m.)) and (right) B6 lung allografts 30 days after transplantation into MR1- and CTLA4-Ig-treated CBA mice (21.77±3.55%) (n=3 each). (b) Immunofluorescent staining of Foxp3+ cells in bronchus-associated lymphoid tissue (BALT) of Balb/c lung grafts 30 days after transplantation into MR1- and CTLA4-Ig-treated B6 CD11c-EYFP recipient. Foxp3-expressing cells are red, CD11c+ dendritic cells are green, and nucleated cells are blue (4′,6′ diamidino-2-phenylindole (DAPI)). (c) Histological appearance and (d) rejection score (International Society of Heart and Lung Transplantation (ISHLT) grade A) for B6 lung grafts that were initially transplanted into immunosuppressed (MR1, CTLA4-Ig) CBA recipient that also received PC61 and then retransplanted into nonimmunosuppressed CBA host shown 30 days after retransplantation (n=4).
