Figure 9
Intravital two-photon imaging of dynamic T-cell behavior within induced bronchus-associated lymphoid tissue (BALT) in accepted Balb/c lung grafts 30 days after transplantation into immunosuppressed (MR1 250 μg intraperitoneally (i.p.) day 0, CTLA4-Ig 200 μg i.p. day 2) B6 CD11c+ EYFP recipients. (a) Time-lapse behavior of adoptively transferred 4-chloromethyl benzoyl amino tetramethyl rhodamine (CMTMR)-labeled B6T cells in dendritic cell clusters with tracks indicating representative T cells. Images are individual frames from a continuous time-lapse movie. Relative time is displayed in h:min:s. (b) Number of extravasated T cells within BALT and adjacent graft tissue. Plots of (c) average track speed (μm min−1) and (d) meandering index of extravasated T cells within BALT. Meandering index was calculated by dividing the distance a cell traveled from its starting point by the track length. (e) Individual T lymphocytes were tracked and the cell displacement squared (μm2) vs. time (min) shows a strong linear correlation indicative of random cell migration. n=35, *P< 0.05, bar=60 μm.
