Figure 2
Induction of interleukin (IL)-10 and type 1 regulatory T (Tr1) cells in lymphocytes by Neisseria gonorrhoeae (Ngo) is partially dependent on both opacity (Opa) proteins and lipooligosaccharide–Toll-like receptor 4 (LOS-TLR4) interactions. (a) IL-10 secretion by iliac lymph node (ILN) cells or purified CD4+ T cells stimulated with wild-type (WT) vs. Opa– N. gonorrhoeae (multiplicity of infection (MOI) 10:1) for 4 days. (b) The percentages of induced Tr1 cells in CD4+ T cells in cultures of ILN cells or purified CD4+ T cells stimulated with WT vs. Opa– N. gonorrhoeae (MOI 10:1) for 4 days. (c) IL-10 secretion and (d) Tr1 cell production by WT (C3H/FeJ) vs. TLR4-deficient (C3H/HeJ) ILN or purified CD4+ T-cell cultures stimulated with N. gonorrhoeae FA1090 (MOI 10:1) for 4 days. Results from one representative out of four independent experiments are shown. #P<0.05; *P<0.01 Opa– vs. WT N. gonorrhoeae or TLR4-deficient vs. WT cells. APC, antigen-presenting cells; Foxp3, forkhead box p3.
