Figure 6
Anti-gonococcal antibody (Ab) responses to primary and secondary infection in anti-interleukin (IL)-10 antibody-treated, anti-IL-10 plus anti-TGF-β (anti-transforming growth factor-β) antibody-treated, control immunoglobulin G (IgG)-treated, or sham-infected BALB/c mice. (a) Vaginal IgA and IgG responses to primary infection. (b) Serum IgA and IgG responses to primary infection. (c) Vaginal IgA and IgG responses to secondary infection. (d) Serum IgA and IgG responses to secondary infection. Vaginal washes and sera were collected 15 days after inoculation, and gonococcus-specific and total IgA and IgG were measured by enzyme-linked immunosorbent assay. Data shown are from one experiment using seven mice per group. #P<0.05; *P<0.01 anti-IL-10 vs. control IgG treatments or anti-IL-10 plus anti-TGF-β vs. anti-IL-10 treatments. Two more replicate experiments yielded essentially similar results and levels of significance. Ngo, Neisseria gonorrhoeae.
