Figure 4
From: Oxidative stress-mediated iNKT-cell activation is involved in COPD pathogenesis
Invariant natural killer T (iNKT) cells have a deleterious function in lung alteration induced by acute exposure to CHP. (a and b) Wild-type (WT) mice were intranasally exposed three times to cumen hydroperoxide (CHP; 75μg per mouse), and 24 h after the last challenge, lung tissues were harvested and processed. The frequency (a) and number (b) of iNKT cells in lung tissues were determined. Data are representative of one experiment out of three performed (mean±s.e.m., n=4), **P<0.01. (c) Expression of CD69 by lung iNKT cells after CHP exposure. (d) Age-matched WT or Jα18−/− mice, either reconstituted or not with WT or Il-17−/− iNKT cells (1 × 106 WT iNKT cells per mouse, i.v. injection) were exposed to CHP. Twenty-four hours after the last challenge, changes in airway resistance were measured in response to increasing doses of methacholine. Results are expressed as the mean±the s.e.m. (n=4) and are representative of three independent experiments. *P<0.05, **P<0.01. (e) Absolute numbers of CD45+ CD11c− F4/80− CD11b+ Ly6C+ neutrophils and CD45+ TCRβ− NK1.1+. NK cells were analyzed among lung mononuclear cells of mice exposed to CHP. Values represent the mean±s.e.m. (n=4), **P<0.01.
