Figure 2
Synergistic induction of interleukin (IL)-33 by IL-4 and H. capsulatum (Hc) in vitro. (a) IL-33 mRNA expression measured by quantitative real-time reverse transcription PCR in wild-type (WT) bone marrow–derived macrophages (BM) that were exposed to IL-4 (10 ng ml−1) or H. capsulatum (5 multiplicity of infection (MOI)) or both for 24 h. All macrophages exposed to IL-4 or IL-4+H. capsulatum were initially primed with IL-4 (10 ng ml−1) for 24 h. Values are normalized to untreated macrophages. All the treatment groups are significantly different (P<0.05) from each other. (b and c) IL-33 mRNA expression in resident peritoneal macrophages (PM) and alveolar macrophages (AM) isolated from WT mice that were exposed to IL-4 (10 ng ml−1) or H. capsulatum (5 MOI) or both in vitro for 24 h. All the treatment groups are significantly different (P<0.05) from each other. (d) Intracellular IL-33 protein content in whole-cell lysates (cells lysed with deionized water) measured by enzyme-linked immunosorbent assay after 24 h of exposure to the stimulus. All the treatment groups are significantly different (P<0.05) from each other. Values depicted in all the above experiments are mean±s.e.m. from at least five independent experiments.
