Figure 3
From: Stromal cells as trend-setters for cells migrating into the lymph node
MAdCAM-1 is induced on donor-derived endothelial cells. EGFP+ stromal cells (SCs) isolated from peripheral LN (pLN) and mesenteric LN (mLN) were transplanted into the mesentery (mes) and popliteal fossa (pop), respectively. Eight weeks later, the newly generated lymph node (LN) were removed and cryosections of the transplanted LN fragments (Neotx-LN) or control LNs were stained for high endothelial venule (HEV; anti-CD31, blue) and MAdCAM-1 (red). HEVs of control mLN were found to be double-positive, similar to HEV in Neotx-pLNmes, whereas control pLN and Neotx-mLNpop showed no MAdCAM-1 staining on HEV. EGFP, enhanced green fluorescent protein.
